Not known Details About thr77
Not known Details About thr77
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in lots of tumors, which results in chromosome instability, and thus might lead to tumorigenesis. This protein was identified to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital position in marketing mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are actually described. Two extra splice variants were claimed but in depth nucleotide sequence data is not yet available.
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Blocking peptides are peptides that bind especially towards the concentrate on antibody and block antibody binding. These peptide ordinarily has the epitope identified via the antibody.
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This guidebook outlines even more probable optimization of cellular and immunoassay parameters to be certain the absolute best results are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to be sure the absolute best effects are...
Antibodies certain to the blocking peptide now not bind on the epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs on the very conserved cyclin loved ones, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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